Phytochemical screening and antimicrobial activity of plant leaf extract against enteric bacterial pathogens

Punica granatum, a fruit-bearing shrub belonging to the Lythraceae family, offers various benefits humans, with both its fibers and seeds being valuable. The contain important oil acids such as palmitic, punica, stearic, oleic acids. In India, P. granatum (pomegranate) leaves have been investigated for their potential antibacterial activity against enteric pathogens. These pathogens are responsible causing gastrointestinal infections often associated antibiotic resistance. study aims explore whether pomegranate possess properties that can combat these effectively. This research holds promise in identifying natural alternative approach address challenges, potentially contributing development of novel antimicrobial agents is commonly used traditional remedy treating bacterial To investigate properties, researchers studied aqueous extract leaf pathogens, conducting tests like biochemical examinations microscopy on isolates. phytochemical analysis revealed presence bioactive compounds. compounds include amino acids, carbohydrates, alkaloids, steroids, flavonoids, terpenoids, saponins, tannins, phenols. evaluate activity, discs, including chloramphenicol, vancomycin, gentamicin, employing standard Kirby Bauer method. Results showed vancomycin had highest zone inhibition, followed by chloramphenicol respective measurements 20 mm 19 mm. Chloramphenicol exhibited sensitivity Escherichia coli (19 mm), while gentamicin Salmonella typhi (20 Staphylococcus aureus E. (18 mm). Vancomycin demonstrated S. (21 displayed significant at concentrations 20, 40 mcg, 60 μg, showing effective zones inhibition ranging from 15 22 Pseudomonas aeruginosa, coli, aureus, Shigella dysentriae, Proteus vulgaris, typhi. conclusion, established extracts remarkable valuable pharmaceutical Due efficacy disorders, it utilized medicine.